Abstract Chickens genetically resistant to avian influenza could prevent future outbreaks. In chickens. influenza A virus (IAV) relies on host protein ANP32A. Here we use CRISPR/Cas9 to generate homozygous gene edited (GE) chickens containing two ANP32A amino acid substitutions that prevent viral polymerase interaction. https://chefesquipmenters.shop/product-category/steak-knives/